浙江省大豆种质资源蛋白亚基构成分析

Analysis on protein subunit contents of soybean germplasm in Zhejiang Province

以杭州国家大豆改良分中心保存的321份浙江省大豆资源为研究材料,利用SDS-PAGE电泳技术,对其贮藏蛋白中7S和11S球蛋白及其亚基的相对含量（占总蛋白比例）进行了系统的分析。结果表明：浙江省大豆资源7S蛋白亚基相对含量变化范围在0.152~0.371;11S蛋白亚基相对含量变化范围在0.428~0.794;11S/7S比值介于1.269~4.008,平均值为2.289,其中蛋白含量高于45.0%,11S/7S比值高于3.5的有7份种质。不同大豆资源蛋白亚基组成和相对含量存在显著差异。相关分析表明,11S与7S蛋白亚基相对含量呈极显著负相关。在试验中还发现4份大豆资源均表现为7S球蛋白α,′α亚基含量较低,并且在30 kD至17 kD间出现未知小分子肽。依据浙江省大豆生态区域划分,浙北地区大豆贮藏蛋白11S/7S比值平均值最高;浙南地区大豆7S和11S相对含量变异系数高于浙北和浙中地区。

Three hundred and twenty one soybean germplasm resources of Zhejiang Province from the germplasm bank of Hangzhou Sub-center of National Soybean Improvement were analyzed to investigate the 7S and 11S globulins contents using sodium dodecyl sulfate-polyacrylamide gel electrophoresis（SDS-PAGE）.The results showed a great genetic diversity in the relative contents of 11S,7S globulins and their subunits.The variation ranges of the relative content of 7S,11S globulins and the 11S/7S ratio were 0.152-0.371,0.428-0.794 and 1.269-4.008 respectively.There were seven germplasms,whose 11S/7S ratios were higher than 3.5,and protein content percent was higher than 45.0%.Four germplasms showed relative lower α′,α-subunit content than others,and some unknown small peptides between 30 kD and 17 kD appeared.Significantly negative correlation was observed between the relative contents of 7S and 11S globulins.The 11S/7S ratios of different germplasms showed regional features according to their ecology regionalism.Germplasms from North Zhejiang showed the highest average 11S/7S ratio,and germplasms from South Zhejiang showed higher coefficient of variation in relative contents of 11S,7S globulins than other areas.