摘要
为阐明灵昆鸡群体遗传变异和遗传结构状况,采用23个家鸡特异性的微卫星标记对该鸡种自然群体中30个个体进行PCR扩增和聚丙烯酰胺凝胶电泳分析,检测其等位基因。结果表明,共检测到174个等位基因,23个座位都呈现出多态性,23个微卫星座位的等位基因数在3~12个,平均等位基因数为7.5个。群体平均杂合度和平均多态信息含量分别为0.758和0.723。分析结果表明灵昆鸡自然群体遗传多样性较丰富。
In order to assess Lingkun chicken＇s genetic variation and population structure,23 chicken-specific microsatellite loci were used to analyze the alleles for 30 individuals from natural population using PCR and polyacrylamide gel electrophoresis.A total of 174 alleles were detected across the 23 microsatellite loci.All the loci were polymorphic and the number of alleles varied from 3 to 12,and the mean number was 7.5 alleles per locus.The average heterozygosity and polymorphism information content of 23 microsatellite loci were 0.758 and 0.723,respectively.The results indicated that there was high genetic diversity in the natural population of Lingkun chicken.
出处
《浙江农业学报》
CSCD
北大核心
2010年第4期458-463,共6页
Acta Agriculturae Zhejiangensis
基金
浙江省重大科技攻关项目(2005C1200501)
关键词
微卫星标记
灵昆鸡
遗传多样性
杂合度
多态信息含量
microsatellite marker
Lingkun chicken
genetic diversity
heterozygosity
polymorphism information content
