摘要
以紫外线和氮离子注入作为诱变手段对曲酸产生菌黄曲霉CICC 2242进行诱变处理,筛选出一株高产菌株N83。曲酸产量由初始的12.4g/L提高到19.4g/L,提高了56.5%;遗传稳定性实验结果表明,N83遗传性状稳定良好。该结果表明氮离子注入诱变是获得高产曲酸的有效途径。
The kojic acid-producing strain Aspergillus flavus CICC 2 242 was mutagenized using UV radiation and N^＋ implantation, and its mutant strain A. flavlts N83 was obtained. The mutant strain produced kojic acid at 19.4 g/L, which was 56.5 % higher than that with A. flavus CICC 2 242 at 12.4 g/L. Moreover, A. flavus N83 showed good genetic stability. The results showed that N^＋ implantation was an effective method to generate better kojic acid producer.
出处
《激光生物学报》
CAS
CSCD
2010年第4期542-545,共4页
Acta Laser Biology Sinica
基金
中国科学院知识创新工程领域前沿项目(S200719)
关键词
曲酸
紫外线
氮离子注入
诱变
kojic acid
UV radiation
N^＋ implantation
mutagenesis
