创伤性休克对大鼠CD4+T淋巴细胞凋亡及分化的影响

Influence of trauma/hemorrhagic shock on CD4+T lymphocyte apoptosis and differentiation in rats

目的严重创伤性休克可致机体炎症及免疫反应失衡,并由此引起严重感染、多脏器功能衰竭。本研究旨在探究继发于创伤性休克(TH/S)的CD4+T淋巴细胞分化及凋亡的改变。方法以乳酸林格氏液对创伤性休克大鼠进行复苏,分别于复苏后12,24,48小时检测CD4+T淋巴细胞的数量,凋亡发生率,向Th1/Th2细胞分化比例以及淋巴细胞的NF-κB活性,设模型组(TH/S)、假手术组(SHAM)及空白组(CONTROL)进行对照研究。结果创伤性休克后的液体复苏能够恢复大鼠血流动力学趋向稳定。模型组相较于假手术组及空白组CD4+T淋巴细胞比例减少(TH/S:23.8±5.4%,SHAM:55.2±2.6%,CONTROL:52.4±3.8%,复苏后48小时),凋亡比例明显增加(TH/S:15.2.4±5.4%,SHAM:7.23±3.4%,CONTROL:8.24±4.3%,复苏后48小时),Th1/Th2细胞比值明显下降(TH/S:0.37±0.12,SHAM:0.71±0.16,CONTROL:0.66±0.23,复苏后48小时),同时脾淋巴细胞的NF-κB活性亦有明显升高(TH/S:0.41±0.09;SHAM:0.17±0.04;CONTROL:0.23±0.05;复苏后48小时);而假手术组与空白组无明显差异。结论创伤性休克可能破坏机体Th1/Th2反应的平衡,同时改变了NF-κBp65活性并促进CD4+T细胞凋亡的发生。

Objective Trauma/hemorrhagic shock（TH/S） has been associated with inflammation and immunodisorders,leading to immunosuppression,multiorgan dysfunction and death.In this research,we plan to observe CD4＋T lymphocyte apoptosis and the balance of Th1 and Th2 responses correlated with TH/S.Methods Male Sprague-Dawley rats with TH/S injury were resuscitated with lactated Ringer solution.The impact of TH/S and on the activation,differentiation and survival of CD4＋T cells was longitudinally examined post resuscitation.Results Following resuscitation,the MAP,as expected,gradually increased toward the baseine.In the group TH/S,compare with group SHAM and CONTROL,the percentage of CD4＋ T cells decreased（TH/S：23.8±5.4%,SHAM：55.2±2.6%,CONTROL：52.4±3.8%）,more CD4＋T cells were induced apoptosis（TH/S：15.2.4±5.4%,SHAM：7.23±3.4%,CONTROL：8.24±4.3%）,and the ratio of Th1：Th2 responses was significantly reduced（TH/S：0.37±0.12,SHAM：0.71±0.16,CONTROL：0.66±0.23）.Moreover,the NF-κB p65 activation in spleen cells was induced by TH/S（TH/S：0.41 ±0.09;SHAM：0.17±0.04;CONTROL：0.23±0.05）.Conclusion These data demonstrated that TH/S and resuscitation destroyed the balance of Th1 and Th2 responses and induced the NF-κB activation and CD4＋ T cell apoptosis in rats.