惊厥大鼠不同脑区突触体内单胺类递质水平的动态变化及意义

Dynamic Changes and Significence of Synaptic Monoamine Transmitter Levels in Different Brain Regions of Epileptic Rats

目的探讨单胺类递质与惊厥的关系。方法选用Wistar大鼠32只,采用腹腔注射硫代氨基脲制作惊厥模型。取大脑皮层、海马、纹状体三个脑区,制备出突触小体。用荧光分光光度计测定五羟色胺(5-HT)、多巴胺(DA)、去甲肾上腺素(NA)三种单胺类递质的含量。结果 5-HT含量在惊厥发作组中,大脑皮层和海马区显著下降(P<0.01),而纹状体仅致死组有明显降低(P<0.01);DA含量在惊厥组中仅纹状体有显著下降(P<0.01);而大脑皮层和海马区无明显变化(P>0.05);NA含量在惊厥组中,仅海马区有明显下降(P<0.01);而大脑皮层则随惊厥初发、再发和致死组反见升高;在纹状体,致死组有明显下降(P<0.05)。结论惊厥的发生、发展与特定脑区突触体内特定的单胺类递质含量有关,并非所有脑区突触体内出现普遍性单胺类递质的下降;不同部位的神经递质与惊厥的敏感性和发作严重程度有关。

Objective To explore the relation between monoamines and epilepsy.Methods The rat model of seizure was made by intraperitoneal injection.The palium（Pal）,hippocampus（Hip）and corpus striatum（Str） were taken immediately and the brain synaptosomes were prepared after the rats were sacrificed.The levels of serotonin（5-HT）,dopamine（DA） and norepinephrine（NA）in synaptosomes were determined by fluorescence spectrophotometer.Results The levels of 5-HT decreased significantly in Pal and Hipin from experimental groups（P〈0.01）,but decreased in Str from lethal group（P〈0.01）.The levels of DA decreased significantly only in Str from convulsive groups（P〈0.01）.The levels of NA markedly decreased only in Hip from convulsive groups（P〈0.01）,While in Pal,it increased with the consequence of incipience,relapse and death.In Str,DA levels decreased markedly only in lethal grop（P〈0.05）.Conclusion The onset and development of epilepsy are related to the levels of certain monoamine in synaptosomes of cerain brain regions.The transmitters in different regions may affect the sensitivity and be related to the severity of epilepsy.