钝顶螺旋藻藻胆蛋白的纯化及其清除氧自由基的作用

Stadies on phycobiliprotein of Spirulina platensis for its purification and ability to scavenge active oxygen radical

本文对钝顶螺旋藻藻胆蛋白（包括藻蓝蛋白，缩写Ｃ－ＰＣ和别藻蓝蛋白，ＡＰＣ）的分离工艺进行了研究，建立经细胞破碎、硫酸铵沉淀蛋白，羟基磷灰石柱分离工艺。经凝胶电泳、吸收光谱、荧光光谱和量热分析等研究，证明 Ｃ－ＰＣ由大小两个亚基组成，分子量分别为 １７ ２００ｕ和 １４ ９００ｕ；ＡＰＣ由一个亚基组成，分子量为１３ ４００ｕ。其最大吸收峰分别在 ６２５ｕｍ和 ６６０ｕｍ处。其荧光发射峰位于 ６４８ｕｍ。通过 ＤＳＣ分析发现，ＡＰＣ的变性温度在 ４９．０℃～６０．５℃范围。应用黄嘌呤氧化酶－黄嘌呤－Ｌｕｍｉｎｏｌ发光体系证实 ＡＰＣ具有对 Ｏ_２－的清除作用。

Two types of phycobiliprotein,C-PC and APC were separated just after the smashed cells were precopitated in (NH_4)_2SO_4 and then loaded on the hydroxylapatite column. The phycobiliprotein' s properties were investigted by methods of electrophoresis, absorption spectrum, fluorescence spectrum and differential scanning calorimetry. The results show that the molecular weights of subunits of the C-PC are 17 200u and 14 900u,APC are 13 400u, Absorption maximum at 625um and 660um,and a room fluorescence emission peak at 648um and 668um. The temperature of denaturation of the APC is in the range of 49. 0 to 60. 5℃. The APC for scaverging active oxygen radical was reported in this paper. The results showed that it could scavenge O_2^-.