沙眼衣原体感染后大鼠蜕膜中TRAIL及受体的表达

Expressions of TRAIL and its receptor in decidual tissues of rats after chlamydia trachomatis infection

目的:通过大鼠生殖道沙眼衣原体(Chlamydia trachomatis,CT)感染模型研究CT初次感染后妊娠大鼠在胚胎着床期子宫蜕膜组织中肿瘤坏死因子相关凋亡诱导配体(tumor necrosis factor related apoptosis-inducing ligand,TRAIL)及其受体1(TRAIL receptor1,TRAIL R1or DR4)的mRNA及蛋白表达,探讨TRAIL/TRAILR在妊娠中的作用。方法:选择成年雌性SD大鼠38只,随机均分为实验组和对照组,实验组孕前阴道成功接种CTD型株。胚胎植入期处死大鼠,计数胚胎植入数;子宫组织分为两份,分别用来逆转录扩增(RT-PCR)半定量检测TRAIL、DR4的mRNA的相对表达量及免疫组化法确定其蛋白表达,SPSS软件处理数据分析差异显著性。结果:①实验组胚胎植入数下降(P<0.01)。②实验组DR4的mRNA高表达,差异有统计学意义(P<0.001);TRAIL的mRNA表达差异无统计学意义(P>0.05);DR4mRNA的表达量与对应的胚胎植入数呈负相关(r=-0.782,P<0.05)。③实验组TRAIL免疫组化阳性颗粒的平均光密度值差异无统计学意义(P>0.05);实验组DR4升高,差异有统计学意义(P<0.01)。结论:雌性大鼠生殖道感染CT后,胚胎植入数减少,这种变化可能与子宫组织DR4的高表达、与TRAIL过量结合导致细胞凋亡过度有关。

Objective：To research the expressions of mRNA and protein of tumor necrosis factor related apoptosis-inducing ligand （TRAIL） and its receptor 1 （TRAIL R1 or DR4） in uterine decidual tissues at implantation period of pregnant rat models with primary infection of chlamydia trachomatis,explore the functions of TRAIL/TRAILR in pregnancy.Methods：38 adult female SD rats were selected and divided into experimental group and control group randomly.The rats in experimental group were vaginally infected with D strain of chlamydia trachomatis;all the rats were sacrificed at implantation period,the number of implanted embryo was counted;the uterine tissues were divided into two subgroups to detect the relative expression levels of TRAIL and DR4 mRNA by RT-PCR and the expressions of TRAIL and DR4 protein by immunohistochemical staining,SPSS software was used in the study.Results：The number of implanted embryo in experimental group decreased significantly （P0.01）.The expression level of DR4 mRNA in experimental group was high,there was significant difference （P0.001）,but there was no significant difference in expression level of TRAIL mRNA between the two groups （P0.05）;there was a negative correlation between expression level of DR4 mRNA and corresponding number of implanted embryo （γ=-0.782,P0.05）.There was no significant difference in average optical density of immunohistochemical positive particles between the two groups （P0.05）;the expression of DR4 in experimental group increased significantly （P0.01）.Conclusion：The number of implanted embryo decreases after reproductive tract infection of chlamydia trachomatis in female rats,the change may be related to excessive apoptosis induced by high expression of DR4 in uterine tissues and its excessive combination with TRAIL.