子痫前期患者胎盘组织IL-4 mRNA和IFN-γ mRNA的表达水平

The expression levels of IL-4 mRNA and INF-γ mRNA in placental tissues of patients with preeclampsia

目的:探讨子痫前期的病因及发病机制。方法:用原位杂交法测定子痫前期患者胎盘组织中IFN-γmRNA和IL-4mRNA的基因表达。结果:①IL-4mRNA在正常妊娠组、子痫前期轻度组和子痫前期重度组的表达随病情的加重表达减弱,但3组间两两比较无统计学意义(P>0.05);②IFN-γmRNA的表达随病情的加重表达增强,子痫前期重度组与正常妊娠组、子痫前期轻度组相比差异有统计学意义(P<0.05),且与正常妊娠组相比差异显著(P<0.001);子痫前期轻度组的表达也高于正常妊娠组,但差异无统计学意义(P>0.05);③IFN-γmRNA/IL-4mRNA比值结果显示,随病情的加重比值增大,且子痫前期轻度组、重度组与正常妊娠组两两相比差异均有统计学意义(P<0.05),重度组与正常妊娠组相比差异显著(P<0.001)。结论:子痫前期孕妇胎盘中表现为免疫杀伤的Th1型细胞因子IFN-γmRNA表达增强,表现为免疫保护或免疫营养的Th2型细胞因子IL-4mRNA表达无差异,提示子痫前期患者母胎界面中IFN-γmRNA的高表达与其发病有关,IL-4mRNA在子痫前期的发生、发展中的作用不明显;IFN-γmRNA/IL-4mRNA比值随病情的加重而增大,提示母胎界面的Th1/Th2细胞因子的平衡偏离可能是导致子痫前期发病的病因之一。

Objective：To explore the pathogenesis and mechanism of preeclampsia.Methods：The gene expressions of IL-4 mRNA and INF-γ mRNA in placental tissues of patients with preeclampsia were detected.Results：The expression of IL-4 mRNA in normal pregnancy group,mild preeclampsia group and severe preeclampsia group decreased with the aggravation of disease,but there was no significant difference among the three groups （P0.05）;the expression of IFN-γ mRNA increased with the aggravation of disease,there was significant difference between severe preeclampsia group and normal pregnancy group,mild preeclampsia group （P0.05）;the expression of IFN-γ mRNA in mild preeclampsia group was higher than that in normal pregnancy group,but there was significant difference （P0.05）;the ratio of INF-γ mRNA/IL-4 mRNA increased with the aggravation of disease,and there was significant difference among the three groups （P0.05）.Conclusion：The high expression of IFN-γ mRNA which plays a role immune killing and the normal expression of IL-4 mRNA which plays a role immune protection or immune nutrition in placental tissues of patients with preeclampsia indicate that the high expression of IFN-γ mRNA at maternal-fetal interface is related to pathogenesis of preeclampsia and the effect of IL-4 mRNA on occurrence and development of preeclampsia is not obvious;the ratio of INF-γ mRNA/IL-4 mRNA increases with the aggravation of disease,which indicated that Th1/Th2 cytokines imbalance may be one of the causes of preeclampsia.