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口腔癌组织中缺氧微环境对单核/巨噬细胞分泌VEGF影响的实验研究 被引量：1

Experimental study on secretion of VEGF of monocytes/macrophages in hypoxic microenvironment in oral cancer
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摘要 目的通过缺氧微环境对单核/巨噬细胞分泌血管内皮生长因子(VEGF)影响的实验研究,探讨口腔癌组织局部微环境对单核/巨噬细胞功能的影响。方法从健康人的外周血中分离单个核细胞并通过培养将其转化为单核/巨噬细胞;分别在缺氧(O2<1%)和常氧条件(20%O2)下加入口腔鳞癌细胞系Tca8113培养上清液进行单核/巨噬细胞培养,在3、5、7 h采用酶联免疫吸附试验(ELISA)法检测培养上清液中VEGF的表达。结果在缺氧微环境下,单核/巨噬细胞分泌VEGF的能力较常氧环境下增加,并随时间的延长而增加(P<0.05)。结论在缺氧的微环境下,单核/巨噬细胞可能更多的通过生长因子的分泌参与肿瘤的生长,但单核/巨噬细胞在肿瘤组织中的作用其具体机制及在肿瘤免疫治疗中的意义还需进一步的研究。 Objective To investigate the secretion of vascular endothelial growth factor（VEGF） of monocytes/macrophages in hypoxic microenvironment of oral cancer in vitro.Methods Peripheral blood mononuclear cells were extracted from healthy person′s blood and cultivated to transform into monocytes/macrophages.Then monocytes/macrophages were cultured in hypoxic microenvironment（O〈21%） and in normal microenvironment（20% O2）.The expression of VEGF was detected by enzyme-link immunoassay（ELISA）.Results The expression of VEGF excreted by mononuclear/macrophage was significantly higher in hypoxic microenvironment than in normal microenvironment.Conclusion In hypoxic microenvironment,the secretion of VEGF by mononuclear/macrophage is gradually increased.But the mechanism of monocytes/macrophages′ function in tumor needs more research.
作者 冯红超 彭江帆 马洪 宋宇峰
机构地区 贵阳医学院口腔医学系口腔颌面外科教研室
出处 《重庆医学》 CAS CSCD 北大核心 2010年第17期2244-2245,共2页 Chongqing medicine
基金 国家自然科学基金资助项目(30660200) 贵州省优秀科技教育人才省长专项基金资助项目(2005)160号
关键词 单核/巨噬细胞 口腔癌 缺氧 血管内皮生长因子 monocytes/mcrophages carcinoma hypoxia VEGF
分类号 R739.85 [医药卫生—肿瘤] R733 [医药卫生—肿瘤]
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参考文献11

  • 1Villanueva J, Herlyn M. Melanoma and the tumor microenvironment[J]. Curr Oncol Rep, 2008,10(5) : 439.
  • 2Collingridge DR, Hill SA, Chaplin DJ. Proportion of infiltrating IgG-binding immune cells predict for tumor hy poxia[J]. Br J Cancer,2001,84(5) :626.
  • 3Elgert KD, Alleva DG, Mullins DW. Tumor-induced immune dysfunction:the macrophage conneetion[J]. J Leukoc Biol,1998,64(3) :275.
  • 4Pollard JW. Tumor-educated macrophages promote tumour progression and metastasis[J]. Nat Rev Cancer, 2004,4 ( 1 ) : 71.
  • 5Gordon S. Alternative activation of macrophages[J]. Nat Rev Immunol, 2003,3 (1) : 23.
  • 6孙乐刚,刘玲,冯红超,宋宇峰.血管细胞粘附分子-1在口腔鳞状细胞癌中的表达及其与血管生成的关系[J].现代口腔医学杂志,2007,21(3):267-270. 被引量：7
  • 7Yuan A,Chen JJ, Yang PC. Pathphysiology of tumor-associated macrophages[J].Adv Clin Chem, 2008,45 : 199.
  • 8Allavena P, Sica A, Solinas G, et al. The inflammatory micro-environment in tumor progression: the role of tumor-associated maerophages[J]. Crit Rev Oncol Hematol,2008,66(1) :1.
  • 9金晓明,李雅馨,刘桂芳,李金荣,李宁毅,贾暮云.血管内皮生长因子对口腔鳞状细胞癌细胞侵袭能力的影响[J].现代口腔医学杂志,2005,19(1):67-69. 被引量：5
  • 10冯红超,宋宇峰.口腔鳞癌巨噬细胞和微血管的空间关系[J].贵阳医学院学报,2003,28(1):12-14. 被引量：7

二级参考文献31

  • 1[1]Leek RD, Lewis CE, Whitehouse R,et al. Association of macrophage infiltration with angiogenesis and prognosis in invasive breast carcinoma[J]. Cancer Res, 1996,56(20):4625-4629.
  • 2[2]Liss C, Fekete MJ, Hasina R, Lam CD,et al. Paracrine angiogenic loop between head-and-neck squamous-cell carcinomas and macrophages[J]. Int J Cancer, 2001,93(6):781-785.
  • 3[3]Ono M,Torisu H,Fukushi J,et al. Biological implications of macrophage infiltration in human tumor angiogenesis[J].Cancer Chemother Pharmacol,1999,43:S69-71.
  • 4[4]Vauple P,Kallionwski F,Okunieff P.Blood flow,oxygen and nutrient supply,and metabolic microenviroment of human tumours:a review[J].Cancer Res,1989,49:6449-6465.
  • 5[5]Brown JM,Giaccia AJ.The unique phydiology of solid tumours:opportunities (and problems)for cancer therapy[J].Cancer Res,1998,58:1408-1416.
  • 6[6]Crowther M, Brown NJ, Bishop ET,et al. Microenvironmental influence on macrophage regulation of angiogenesis in wounds and malignant tumors[J].J Leukoc Biol,2001,70(4):478-490.
  • 7[7]Negus RP,Stamp GW,Relf MG,et al.The detection and localization of monocyte chemoattractant protein-1in human ovarian cancer[J].J Clin Invest,1995,95:2391-2396.
  • 8[8]Walter S,Bottazzi B,Govoni D,et al.Macrophages infiltration and growth of sarcoma clones expressing different amount of monocyte chemotactic protein[J].Int J Cancer,1991,49:431-435.
  • 9[9]Lewis JS,Landers R,Understood JCE,et al.Expression of VEGF by macrophares is upregulated in avascular areas of breast carcinomas[J].J Pathol, 2000,192:150-158.
  • 10Bussolino F, Mantovani A, Persico G. Molecular mechanisms of blood vessel formation. TIBS, 1997,22(3) :251 - 260.

共引文献16

同被引文献8

  • 1宋宇峰,冯红超,温玉明.口腔鳞状细胞癌中巨噬细胞浸润和肿瘤生长、转移的关系[J].中华口腔医学杂志,2005,40(5):385-385. 被引量：9
  • 2彭娟,丁童,郑利民,邵建永.肿瘤相关巨噬细胞对鼻咽癌进展和预后的影响[J].癌症,2006,25(11):1340-1345. 被引量：17
  • 3Takanami I, Takeuchi K, Kodaira S. Tumor-associated macro phage infiltration in pulmonary adenocarcinoma:association with angiogenesis and poor prognosis[J].Oneology, 1999,57 (2) :138.
  • 4Eoffelt S B, Hughes R, Lewis C E. Tumor associated macropha ges effectors of angiogenesis and tumor p rogression[J]. Biochim Biophys Acta,2009,1796(1) : 11.
  • 5Lindsay TH,Jonas BM,Sevcik MA,et al. Pancreatic cancer pain and its correlation with changes in tumor vasculature, macro- phage infiltration, neuronal innervations, body weight and disease progression[J]. Pain, 2005,119 ( 1-3) : 233.
  • 6Chen J J, Lin YC, Yao PL, et al. Tumor-associated macrophages the double edged sword in cancer progression [J]. J Clin Oncol 2005,23(5) ,953.
  • 7Melichar B, Say tivity of ascetic ma[J]. Int J G ary CA,Patenia R. Phenotype and antitumor ac fluid monocytes in patients with ovarian careino ynecol Cancer,2003,13(4):435.
  • 8万挺,刘继红,郑利民,蔡木炎,丁童.肿瘤相关巨噬细胞在晚期上皮性卵巢癌组织中的浸润及其与预后的关系[J].癌症,2009,28(3):323-327. 被引量：24

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重庆医学
2010年 第17期

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