血必净对窒息大鼠心肺复苏后免疫因子及补体变化的实验研究

Study of leverl of complement receptor and immune factor after anoxia-induced cardiac arrest-cardiopulmonary resuscitation treatment by Xuebijing in rats

目的探讨血必净对大鼠心肺复苏后免疫因子及补体水平变化的影响。方法建立心肺复苏大鼠模型,将42只SD大鼠随机分为4组。A组:假手术组(仅进行麻醉和气管切开插管、血管穿刺,不进行窒息及心肺复苏);B组:常规复苏组(常规复苏+生理盐水4 mL/kg),C组:血必净低剂量治疗组(血必净2 mL/kg+生理盐水2 mL/kg);D组:血必净高剂量治疗组(血必净4 mL/kg);动态观察血清补体C3(C3)、补体C4(C4)、白介素-12(IL-12)、白介素-4(IL-4)、肿瘤坏死因子-α(TNF-α)水平的变化。结果与A组比较:B、C、D组IL-12、TNF-α水平复苏后均高于A组(P<0.01);B组复苏后6 h与0 h比较,C3差异有统计学意义(P<0.05),C、D组均差异无统计学意义(P>0.05),各组间C4均差异无统计学意义(P>0.05)。与同组0 h比较:B、C、D组复苏后6 h IL-12、TNF-α水平均升高(P<0.01);C3、C4B组复苏后6h分别下降45.24%,49.97%,且差异有统计学意义(P<0.01),C、D组差异无统计学意义(P>0.05)。与B组比较:C、D组复苏后IL-12、TNF-α水平低于B组(P<0.01),C3、C4水平差异无统计学意义(P>0.05)。在整个实验过程均未能检测到IL-4的浓度。结论心肺复苏后存在补体水平的消耗,炎性因子TNF-α、IL-12过度表达,血必净可减轻复苏后补体水平的消耗及TNF-α、IL-12过度表达。

Objective To study the lever of complement receptor and immune factor after anoxia-induced cardiac arrest-cardiopulmonary resuscitation（CA-CPR） by Xuebijing injection.Methods To establish the CA-CPR model with 42 SD rats,randomly divided into 4 groups.Group A：sham operation group（only anesthesia and tracheotomy intubation,vascular puncture,without suffocate and cardio-pulmonary resuscitation）;group B：conventional resuscitation group（conventional recovery ＋ physiological saline 4 ml/kg）;group C：Xuebijing low-dose treatment group（Xuebijing 2ml/kg）;group D：Xuebijing high-dose treatment group（Xuebijing 4 mL/kg）.To observe the changes of complement receptor C3（C3）,C4,IL-12,IL-4,tumor necrosis factor-ɑ（TNF-ɑ）at any time.Results Compared with group A,the level of IL-12,TNF-α in group B,C,D was higher after CPR（P〈0.01）,the level of C3 on 6 h in group B had the statistic differnece,but there was no statistitical difference between the group C and D;C4 had no statistic significance among these groups（P〉0.05）.Compaired with 0 h in the same groups,the level of IL-12,TNF-α was increased after 6 h in the group B,C,D（P〈0.01）;but the lever of C3,C4 on 6 h in group B was decreased by 45.24% and 49.97%,with the statistic significance（P〈0.01）,there was no statistic difference between the group C and D.Compared with group C,the level of IL-12,TNF-α after CPR in group C,D was decreased（P〈0.01）,but C3,C4 had no statistic significance.Concentration of the factor of IL-4 was not detected during all the experiments.Conclusion There exsits the consumption of complement receptor and the over-expression of inflammation factors including IL-12,TNF-α,and Xuebijing can rectify them.