人参皂苷Rb_1在大鼠肠内菌代谢物吸收入血成分的研究

STUDY ON THE ABSORBED COMPONENTS IN SERUM AFTER ORAL ADMINISTRATION OF GINSENOSIDE Rb 1 IN RATS

目的：研究口服人参皂苷Ｒｂ１（ＧＲｂ１）被吸收入血的成分。方法：给大鼠ｉｇＧＲｂ１５００ｍｇ·ｋｇ－１后，于不同时间采集粪、尿及血清样品，用电喷雾质谱（ＥＳＩＭＳ）检测吸收入血成分。结果：在血与尿中发现分子量为１１０８，９４６及７８４ａｍｕ的代谢产物。经ＥＳＩＭＳ２级质谱分析，上述分子量的化合物分别为ＧＲｂ１，Ｒｄ和Ｆ２。结论：ＧＲｂ１给大鼠ｉｇ后，原形及中间代谢产物Ｒｄ及Ｆ２被吸收入血。

AIM: The experiment was carried out in rats to study the real active component in serum after oral administration of G Rb 1 500 mg·kg -1 . METHODS: The metabolites in serum and urine samples were detected using electron spurt ion mass spectroscopy(ESI MS). RESULTS: Metabolites with molecular weight 1108, 946 and 784 (amu) were shown to be corresponding to Rb 1, Rd and F 2, respectively. CONCLUSION: Rb 1 and its metabolites Rd and F 2 were absorbed into serum after oral administration of Rb 1.