细菌对抗生素最低抑菌浓度不确定性的研究

STUDIES ON NONDETERMINATION OF BACTERIAL ANTIBIOTIC MIC

用绿脓杆菌波动潜生体，羧苄西林诱导潜生体和繁殖体进行抗生素最小抑菌浓度（ＭＩＣ）试验。将奇异变形杆菌和大肠杆菌ｐＨＢ１０１接种于含氨苄西林（ＡＭＰ）的波动培养基上，动态测定培养基中ＡＭＰ变化。观察绿脓杆菌波动生长和奇异变形杆菌寄宿波动中细菌对抗生素ＭＩＣ的不确定性现象并探讨其产生机制。结果表明：绿脓杆菌波动潜生体、羧苄西林诱导潜生体的ＭＩＣ值比繁殖体高８～１６倍。在寄宿波动中，奇异变形杆菌能在高于自己ＭＩＣ值的ＡＭＰ波动培养基上向外扩散，波动出两环。因此，细菌由于经历不同，生理状态也不同，因而对抗生素的ＭＩＣ值表现出不确定性。

To observe the nondetermination of bacterial antibiotic minimal inhibitory concentration (MIC) in hostdependent biowave and waving growth, and its mechanism is discussed. Antibiotic MIC tests were carried out with waving cryptic growth cells (CGC), antibiotic induced CGC and vegetable cells of Pseudomonas aeruginosa (PA). Inoculate Proteus mirabilis (PM) and Escherichia coli (strain pHB101) were inoculated on medium containing ampicilin (AMP) for waving culture, at same time, the changes in AMP was assayed in midium dynamically. The MIC values of PA waving CGC and antibiotic induced CGC were 816 times as much as PA vegetable cells. In hostdependent biowave, PM could disseminate outward displaying two biowave rings, with the medium containing AMP of higher value than PM antibiotic MIC value. Because of different ecosystem, bacteria are in different physiological states, thus their antibiotic MIC values appear nondeterminative.