HLA-A~*02等位基因分型技术及汉族HLA-A~*02多态性研究

A DEVELOPMENT OF HIGHRESOLUTION TYPING FOR HLAA*02 ALLELES AND DETERMINATION OF HLAA*02 PLOYMORPHISM IN THE HANS

建立高分辨率的套式ＰＣＲ－ＳＳＰ，对１７个ＨＬＡ－Ａ＊０２等位基因分型。利用国际标准分型细胞进行特异性鉴定，并在５４例Ａ＊０２阳性的无血缘关系汉族健康人中应用。共检测到５个Ａ＊０２亚型，显示出较高多态性：其中Ａ＊０２１１（ｎ＝２）首次在东方人群检测到；Ａ＊０２０１频率最高，占有４８．２％；其余依次为Ａ＊０２０６、Ａ＊０２０７和Ａ＊０２０３。套式ＰＣＲ－ＳＳＰ既适用于移植供体选择，又可用于人类进化研究。

A nested PCRSSP was developed for the determination the 17 known HLAA*02 alleles. The specificity of the reaction was tested by a panel of international standard genomic DNAs. The method was applied to 54 nonbloodrelated healthy individuals recruited from Chinese Han population positive for A*02 alleles. Five A*02 subtypes were found indicating there was, relative polymorphism among the Han individuals. A*0211 (3.7%), which occurred at a low frequency of 1.3% in the Caucasians, was detected in the Orient for the first time. A*0201 ranked first in the incidence (48.2%). A high proportion of A*0206 A*0203 and A*0203 were also found. The nested PCRSSP could be used as a tool both for the study of human evolution and for HLA matching before tissue or organ transplantation, especially unrelated donorrecipient transplantation.