P27,P73和CyclinD1蛋白在子宫内膜癌中表达的相关性

Expression and Correlation of P27,P73 and CyclinD1 in Endometrial Carcinoma

目的:探讨P27,P73和CyclinD1在子宫内膜癌组织中的表达及其相关性。方法:采用免疫组化SP法对14例正常增殖期子宫内膜、19例不典型增生内膜以及43例子宫内膜癌组织中P27,P73和CyclinD1蛋白的表达进行研究。结果:在子宫内膜癌中,P27表达降低或缺失,P73和CyclinD1表达明显增高,与正常增殖期子宫内膜和不典型增生内膜相比差异有统计学意义(P<0.05或P<0.01)。P27的表达缺失、P73的过表达与组织学分级有关,CyclinD1的高表达与手术-病理分期有关。有肌层浸润者P27,P73和CyclinD1的表达与无肌层浸润者差异均有统计学意义(均P<0.05)。有淋巴结转移者P73和CyclinD1均明显高表达。子宫内膜癌中,P27与CyclinD1呈负相关(r=-0.3627,P<0.05),P73与CyclinD1呈正相关(r=0.3923,P<0.05)。结论:子宫内膜癌中P27表达缺失,P73和CyclinD1过度表达,联合检测可能成为子宫内膜癌高危人群早期筛查、判断内膜癌的生物学行为及评估预后的参考指标。

Objective：The objective is to study the expressions of P27,P73 and CyclinD1 in endometrial carcinoma and the correlation of them. Methods： 14 cases of normal proliferative endometrium, 19 eases of atypical hyperplasia and 43 eases of endometrial carcinoma were detected for the expression of P27 ,P73 and CyclinD1 by means of immunohistoehemieal SP. Results： In endometrial carcinoma, P27 reduced or lowexpressed,and P73 and CyclinD1 overexpressed, which were statistically significant with the expressions in normal proliferative endometri- um and atypical hyperplasia group （P 〈0.05 or P 〈0.01 ）. Lowexpression of P27 and overexpression of P73 were relat- ed to histological grade,while overexpression of CycinD1 were ralated to FIGO stages. The abnormal expression of P27 ,P73 and CyclinD1 in deep invasion was significant difference with that in superficial invasion （P 〈0.05 ）. In the samples with lymph metastasis, P73 and CyclinD1 both overexpressed. In endometrial carcinoma,there was a oppo- site correlation between P27 and CyclinD1 （r =-0.362 7, P 〈0.05）, while there was a positive correlation between P73 and CyelinD1 （r =0.392 3, P 〈0.05 ）. Conclusion： So we concluded that, in endometrial carcinoma, there were lowexpression of P27 and overexpressions of P73 and CyclinD1. ff detected together,the levels of P27,P73 and CyclinD1 proteins may be molecular markers for early screening of high-risk groups, and to determine the biological behavior of endometrial carcinoma and prognosis in reference.