摘要
目的探讨整合素-α2(ITGA2)基因C807T和整合素-β3(ITGB3)基因T176C多态性与缺血性脑卒中的关系及其对血脂、脂蛋白水平的影响。方法应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)和DNA测序的方法检测265例缺血性脑卒中患者和280例对照组ITGA2和ITGB3的基因型;同时按常规方法测定血浆脂质、脂蛋白水平。结果缺血性脑卒中组总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白-胆固醇(LDL-C)水平明显高于对照组(P<0.05),ITGB3基因T176C多态性在缺血性脑卒中组和正常人群中的分布差异无显著性(P>0.05),而ITGA2基因C807T多态性在两组人群中的分布差异存在显著性(P<0.05),等位基因频率的相对风险分析发现,T等位基因携带者患缺血性脑卒中的风险是C等位基因的1.455倍(OR=1.455,95%CI:1.134-1.866),携带T等位基因的缺血性脑卒中个体血浆TC水平显著高于不携带者(P<0.05)。结论 ITGA2基因C807T多态性与缺血性脑卒中的发病具有相关性,其中T等位基因可能是缺血性脑卒中的遗传易感基因;ITGA2基因C807T多态性可能通过影响血脂水平而影响缺血性脑卒中的发生。
Objective To study the relationship between integrin alpha-2 （ITGA2） gene C807T, integfin beta-3 （ITGB3） gene T176C polymorphisms with ischemie stroke and the effect of integrin gene polymorphisms on plasma lipid and lipoprotein levels. Methods Polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） and DNA sequencing were used for the detection of integrin genotypes in 265 patients with ischemic stroke and 280 healthy controls. The plasma lipid and lipoprotein levels were measured by routine method. Results Plasma total cholesterol （TC）, Triacylglycerol （TG） and low density lipoprotein-cholesteml （LDL-C） of the patients with ischemic stroke were significantly higher than those of the controls（ P 〈 0.05） ,The distributions of 1T- GB3 gene T176C polymorphism were not different between ischemic stroke group and control group, but the ITGA2 gene C807T poly- morphism was significantly different. The relative risk suffered from ischemic stroke of C allele was 1. 455 times of the T allele （OR = 1.455,95%CI：1.134- 1.866）,The level of plasma lipid T allele carriers was significantly higher than no carriers （ P 〈 0.05）. Conclusion ITGA2 gene C807T polymorphism was associated with ischemic stroke, 807 T allele may be genetic susceptibility gene for isehemic stroke. ITGA2 gene C807T polymorphism may affect iscbemic stroke through plasma lipid and lipoprotein levels.
出处
《中国实验诊断学》
北大核心
2010年第8期1234-1237,共4页
Chinese Journal of Laboratory Diagnosis
