用于生物量浓度在线检测的光纤衰减全反射传感器

Optical fiber attenuated total reflection sensor for on-line measurement of biomass concentration

为了在生物膜滴滤塔降解有机废气过程中实现生物量浓度的测量,设计了光纤衰减全反射(ATR)生物量浓度传感器,给出了传感器组成原理、结构参数、光路分析及其理论解释。根据光波进入光密介质(Si晶体)与光疏介质(生物菌液)的分界面时所产生的隐失波现象,以及生物菌液对隐失波的散射和吸收引起接收光能量变化的物理机理,建立了一种新的生物量浓度测量方法。实验选用可见光源,在25℃恒温情况下对生物量浓度测量进行了实验研究。实验结果表明:测量结果的相对误差均值为2.217%,基本上满足生物量浓度在线测量要求。该传感器的设计原理和方法具有一定的普遍意义,可应用于制剂、制酒、生化等行业。

With the aim to measure the biomass concentration in the biodegradation of organic waste gas for a trickling biofiher, an optical fiber Attenuated Total Reflection（ATR） sensor for measuring the biomass concentration was designed and the composition principle, structural parameters, optical path analysis and the theoretical explanation of the sensor were also discussed. A new method to meas- ure the biomass concentration was proposed based on the principles that the evanescent wave is scattered and absorbed by the bacterial suspension when the beams enter into the interface between the optically denser medium-Si crystal and the optically less dense-bacterial suspension, and the receiving bacterial power of the light is changed by the bacterial suspension. Under a temperature of 25℃, the biomass concentration was measured by a visible light source. Experimental results indicate that the mean relative error by proposed method is 2. 217%, which can satisfy the on-line measurement requirements of the biomass concentration. The design principle and method of the sensor is significance and can be used in reagent preparation, alcohol production, biochemistry, etc.