DNA倍体分析与脱落细胞学检测对恶性胸腹水诊断的比较

Compared study on DNA ploidy analysis and exfoliative cytologic examination in diagnosis of malignant pleural effusion and ascites

目的比较DNA图像分析系统(ICM-DNA)倍体分析及脱落细胞学检测对恶性胸腹水的诊断价值。方法以158例患者的胸腹水作DNA倍体分析和脱落细胞学检测,比较二者之间的敏感性和特异性。结果 ICM-DNA检测的敏感性为72.50%(58/80),脱落细胞学检查的敏感性为47.50%(38/80),ICM-DNA的敏感性高于脱落细胞学检查,差别有统计学意义(P<0.01);ICM-DNA检测和脱落细胞学检查特异性分别为93.59%(73/78),96.15%(75/78),二者比较差别无统计学意义(P>0.05)。结论 DNA倍体分析联合脱落细胞学检测对于诊断恶性胸腹水有重要意义。

Objective To compare the value of DNA ploidy analysis by DNA image cytometry（ ICM-DNA） and exfoliative cytologic examination in diagnosis of malignant pleural effusion and ascites. Methods ICM-DNA and exfoliative cytology were used to detect one hundred and fifty-eight patients with peural effusion and ascites and their sensitivity and specificity were compared. Results The sensitivity of ICM-DNA and exfoliative cytology were 72. 50% （ 58 /80） ,47. 50% （ 38 /80） respectively,there was significant difference between them （ P〈 0. 01） . The specificity of ICM-DNA and exfoliative cytology were 93. 59%（ 73 /78） ,96. 15% （ 75 /78） respectively,there was no significant difference （ P 〉0. 05） . Conclusion The combination of ICM- DNA ploidy analysis and exfoliative cytologic examination can provide the great significance for differential diagnosis of malignant pleural effusion and ascites.