大鼠脑挫伤后微管相关蛋白2 mRNA表达变化

Expression of Microtubule Associated Protein-2 mRNA after Cerebral Contusion

目的观察大鼠脑挫伤后脑组织中微管相关蛋白2(microtubule associated protein 2,MAP-2)mRNA表达变化,探讨其时序性变化与脑损伤时间的相关性。方法参照Feeney法建立大鼠右顶叶脑挫伤模型,应用实时定量PCR技术检测挫伤区脑组织MAP-2 mRNA的表达情况。结果对照组大鼠脑组织内有低水平的MAP-2 mRNA表达;伤后1h,挫伤灶脑组织MAP-2 mRNA表达较正常对照组下降,伤后6 h降至最低;12 h～14 d逐渐增高,至14 d时仍维持在高表达水平。结论脑挫伤后MAP-2 mRNA的表达规律,在法医学实践中可以作为脑损伤时间推断的新方法之一。

Objective To explore the mRNA expression of microtubule associated protein-2（MAP-2） after cerebral contusion,and to investigate its relationship with post-injury interval in a rat model of cerebral contusion.Methods Based on Feeney’s model of cerebral contusion,the time-dependent changes of MAP-2 mRNA were detected by real-time PCR method in different groups.Results MAP-2 mRNA showed a lower expression in the brain of the control group.The expression of MAP-2 mRNA decreased 1 h post-injury,and reached the lowest level at 6 h,and the expression of MAP-2 mRNA gradually in-creased in the focus of the cerebral contusion from 12 h to 14 d after contusion,which kept a high level up to 14 d post-injury.Conclusion The time-dependent changes of MAP-2 mRNA expression may be a new method for estimating the wound age of cerebral contusion in forensic practice.