Micro-XRF法检测肺内抗酸性硅质颗粒及其在溺死诊断中的应用

Determination of Acid-resistant Silicic Particles in Lung by Micro-XRF and Its Application in Diagnosis of Drowning

目的利用微束X射线荧光光谱(microbeam X-ray fluorescence,Micro-XRF)分析技术检测肺内抗酸性硅质颗粒,并探讨其在法医学溺死诊断中的应用价值。方法将32只大白兔随机分为溺死组(n=12)、死后入水组(n=10)和对照组(n=10),分别提取现场水样和各组肺组织。采用Micro-XRF法测定现场水样和肺组织中抗酸性硅质颗粒的面积含量。结果现场水样中抗酸性硅质颗粒面积含量为4.4mm2/mL;溺死组、死后入水组、对照组肺组织中抗酸性硅质颗粒面积含量分别为(25.30±10.95)mm2/g、(1.68±0.63)mm2/g、(1.65±0.85)mm2/g;溺死组肺组织中抗酸性硅质颗粒面积含量较死后入水组、对照组差异有统计学意义。结论肺内抗酸性硅质颗粒面积含量可作为一种溺死诊断的指标,Micro-XRF测定法灵敏度高、分析速度快,在法医学溺死诊断中具有潜在的应用价值。

Objective Using microbeam X-ray fluorescence（Micro-XRF） analyzer for determination of acid-resistant silicic particles in lung,and to explore its potential application in diagnosis of drowning.Methods Thirty two white rabbits were divided randomly into drowning group（n=12）,post-mortem immer-sion group（n=10） and control group（n=10）.Lungs and water sample were collected for determination of area concentration of acid-resistant silicic particles using Micro-XRF method.Results The area concentra-tion of acid-resistant silicic particles for the drowning water sample was 4.4 mm^2/mL.For the lungs of drown-ing group,the post-mortem immersion group and the control group,the determined average values were（25.30±10.95） mm^2/g,（1.68±0.63） mm^2/g and（1.65±0.85） mm^2/g,respectively,with a statistically significant difference between the drowning group and the other two groups.Conclusion The area concentration of acid-resistant silicic particles in lungs may be used as an indicator of drowning.The method is highly sensitive and rapid.It provides a potential application in drowning diagnosis.