布比卡因在生物样品中的分解动力学

Decomposition Kinetics of Bupivacaine in Biological Specimens

目的研究布比卡因麻醉致死犬的脑组织、血液和尿液在不同保存条件下的布比卡因分解动力学。方法将布比卡因蛛网膜下腔麻醉致死犬的脑组织、血液和尿液分三等份分别置于20℃、4℃和-20℃条件下保存,于死后不同时间点用气相色谱仪检测布比卡因的含量,WinNonlin软件拟合分解动力学方程,计算不同保存条件下脑组织、血液和尿液中布比卡因的分解半衰期。结果脑组织、血液和尿液中布比卡因的分解动力学均符合一级动力学过程,可用lgC=lgCo-kt/2.303表示,式中k为一级分解速率常数。结论 20℃、4℃和-20℃条件下保存的脑组织、血液和尿液中布比卡因均可发生分解,20℃保存时分解较快,其次为4℃,-20℃保存时分解最慢。

Objective To study on the decomposition kinetics of bupivacaine in brain,blood and urine,which were collected from dogs executed by bupivacaine and stored in different conditions.Methods Dogs were given arachnoid cavity anesthesia with bupivacaine.Then the brain,blood and urine were collected and divided equally to three groups stored in 20,4 and-20 ℃ respectively.The concentrations of bupiva-caine at different days were determined by the GC.The equation and half-time period of decomposition kinetics were imitated and calculated with WinNolin program.Results The decomposition kinetics of bupi-vacaine in the dogs’ brain,blood and urine were fit to the first order kinetics.The common equation was lgC=lgCo-kt/2.303 and k was the decomposition constant of first order reaction.Conclusion Bupivacaine in the brain,blood and urine specimens were found to be decomposed at various environments for storage.The higher temperature for storage,the faster of decomposition reaction.