S/O/W法制备PLGA/TiO_2载药微球

PLGA/TiO2 drug loaded microsphere prepared with S/O/W method

将纳米TiO2吸附溶液中的BSA由水包油包固体乳化法用PLGA包裹药物粒子。通过紫外和SEM等检测手段,考察纳米TiO2在不同条件下对BSA吸附率的影响,以及不同制备工艺下PLGA包裹药物粒子对载药量和包封率及体外释放的影响。研究结果表明,当溶液的pH值接近蛋白质等电点、温度在20℃左右、反应24 h时,TiO2吸附BSA量最大,药物粒子分布均匀,尺寸在40 nm左右。在制备微球时,当初乳体积降低、PVA浓度降低及PLGA与药物粉末的质量比增大时,载药量和包封率都有提高,且微球体外释放较小。微球表面光滑,尺寸为4μm左右。

BSA from nano-TiO2 absorbing solution is coated with PLGA via S/O/W method.Using UV-Vis and TEM,the impacts of nano-TiO2 on the BSA absorbing rate under different conditions and influence of PLGA coated drug particles on coating rate and the in-vitro-release are studied.The results show that the largest BSA absorbing on the TiO2 occurs with the conditions： pH is near the isoelectric point of BSA,temperature 20 ℃,incubation time 24 h.The drug article size is even with 40 nm diameter.When the microspheres are prepared,both the drug loaded quantity and coated rate are improved when the volume of primary emulsion and PVA concentration decrease and quality rate of drug powders increase.The 4 μm-diameter microspheres,with smooth surface have small in-vitro-release.