灌服川续断水煎液对大鼠正畸牙移动及牙周组织中破骨细胞的影响

Effects of the traditional Chinese drug Dipsacus asperoides on the reconstruction of paradentium during the orthodontic tooth movement in tats

目的:研究中药川续断在大鼠正畸牙牙周组织改建中的作用机制及其对破骨细胞的影响。方法:选取48只SPF级Wistar雌性大鼠,随机分为川续断组和对照组,每组24只,建立大鼠正畸牙移动实验模型,川续断组每天灌服6g/kg川续断水煎剂,对照组每天灌服3mL生理盐水,2组动物均于正畸加力7、14、21、28d时分批次处死,每批次5只。分离大鼠上、下颌骨,测量牙移动的距离,同时制作上颌第一磨牙区牙周组织切片,光镜观察,并利用PASWstatistics18软件进行数据处理,分别进行t检验和方差分析。结果:川续断组牙移动距离明显大于对照组,差异有统计学意义(P<0.05)。光镜显示,川续断组牙周组织中破骨细胞数目明显增多,与对照组差异有显著(P<0.05)。结论:川续断水煎液能促进大鼠正畸牙移动过程中破骨细胞的增殖和分化,促进牙槽骨吸收及其修复重建,有利于正畸牙移动。

PURPOSE： To observe the effects of the traditional Chinese drug Dipsacus asperoides on the construction of paradentium as well as the mechanism of action to osteoclast during the orthodontic tooth movement in rats.METHODS： Forty-eight female 8-week-old SPF Wistar rats were selected.They were randomly divided into 2 groups： Dipsacus asperoides group and control group.Then animal model for orthodontic tooth movement was established.3 mL of NS was drenched to the control group and the Dipsacus asperoides group was given 6g/kg per day Dipsacus asperoides decoction.The rats were executed in batch on the 7th,14th,21st,28th day during the orthodontic treatment.The distances of the tooth movement were measured and slices from the periodontium of the maxillary first molar were observed under optical microscope.Measurement data were compared with t test and analysis of variance by PASW statistics18.RESULTS： The movement distance of the first molar was significantly larger in the Dipsacus asperoides group than that in the control group.The amount of osteoclast in Dipsacus asperoides group increased significantly compared with that in the control group（P〈0.05）.CONCLUSIONS： Dipsacus asperoides decoction is useful for the proliferation and differentiation of the osteoclast in orthodontic tooth movement.