SEC-10在秀丽线虫逆行行为中功能的研究

Function of SEC-10 in Reversal Behavior of Caenorhabditis elegans

秀丽线虫(Caenorhabditis elegans)是一种研究行为和神经系统相互关系的良好的模式生物,可以在实验条件下给予它物理、化学等多种外部刺激,也可以在分子水平改变其基因结构以改变线虫的行为方式,从而进一步研究神经系统中与行为相关的分子机制.以sec-10突变为遗传背景,采用一种秀丽线虫逆行行为的简单测定方式,研究发现sec-10突变导致线虫逆行动作增多,初步探讨了在突变体线虫的神经系统中重新表达野生型SEC-10可以回复该逆行行为缺陷.

Caenorhabditis elegans is an attractive model organism for studying the relationship between behavior and nervous system.Its behavior can be changed either by external physical or chemical stimuli,or by change of genomic structure,to explore the molecular mechanism of worm behavior in neuronal cells.Here a simple assay was proposed to test the reversal behavior of worms in different genetic background.For the sec-10 mutant worms,it was found that sec-10 mutation led to increased reversal behavior.Further,the reversal defect of sec-10 mutant strain was well rescued by reintroducing wild-type SEC-10 to nervous system.