TERT对小鼠EL-4淋巴瘤细胞增殖调控的初步研究

Study on TERT Regulation for Cell Proliferation in EL-4 Lymphoma Cells of Mice

为了研究端粒酶催化亚基TERT与细胞周期相关基因的调控关系及其在肿瘤细胞增殖中的调控作用,应用基因芯片及RT-PCR等技术,对靶向mTERT的RNA干涉后的小鼠EL-4淋巴瘤细胞进行细胞周期相关基因的表达谱分析,筛选到43个基因在TERT受抑制前后存在表达差异,并且全部为下调基因.表明在小鼠EL-4淋巴瘤细胞中内源性TERT的表达抑制,导致了调控G1期和S期进程的相关细胞周期相关基因的表达变化,并可能通过此途径影响肿瘤细胞的生长和增殖.

In order to investigate the relationship between TRET and cell cycle related genes and the regulative functions of TERT in tumor cells of mice,techniques of gene chip and RT-PCR were used to analyze the expression of cell cycle related genes after RNA interference targeted mTERT in EL-4 lymphoma cells of mice.According to the result of gene chip analysis,among the 96 cell cycle genes of mice,43 genes show significant expression difference between the samples treated with TERT specialized shRNA and the contrast,and all of them belong to the down-regulated genes.The results indicated that the repression of TERT expression in the tumor cells of mice actually leads to the expression change of cell cycle genes which may regulate the G1 and S period,and through this way it influences the growth and proliferation of EL-4 lymphoma cells in mice.