摘要
目的:研究粒细胞集落刺激因子(G-CSF)对脑出血大鼠神经保护作用的机制。方法:胶原酶定位注射构建脑出血大鼠模型,然后给予G-CSF腹腔注射连续5d,用Hoechst33258荧光染色的方法检测G-CSF的抗凋亡作用。结果:术后给予G-CSF的大鼠出血灶所在大脑半球神经细胞凋亡明显低于未给药组。结论:G-CSF在脑出血大鼠脑内具有抗凋亡作用,该作用可能参与其对脑出血大鼠的神经保护作用。
Objective： To study the possible mechanisms underlying the neuroprotective effect of G-CSF in a rat model of ICH. Methods： Experimental ICH was induced by the stereotaxie administration of collagenase type Ⅶ into caudate nucleus. The recombinant human G-CSF（ 15μg/kg）, dissolved in normal saline, was administered through intraperitoneal injection 24h after ICH induction and daily afterwards for 5 days. Then we observed apoptosis by the immunofluoreseenee staining of Hoechst33258. Results：We found that intraperitoneal injection of G-CSF for 5 days could efficiently reduce the number of apoptotic ceils in the hemorrhagic hemisphere of the rats. Conclusions： G-CSF had anti-apoptotic effect after ICH, which may be one of the mechanisms underlying its neuroproteetive effect in a rat model of ICH. KEY WORDS Granulocyte colony-stimulating factor, Intracerebral hemorrhage, Apoptosis
出处
《医学理论与实践》
2010年第9期1044-1045,共2页
The Journal of Medical Theory and Practice
关键词
粒细胞集落刺激因子
脑出血
凋亡
Granulocyte colony-stimulating factor, Intracerebral hemorrhage, Apoptosis
