纳米羟基磷灰石对重金属污染土壤的修复及其对微生物群落结构的影响

Remediation of Heavy Metal Contaminated Soil by Nano-hydroxyapatite and Its Impact on Microbial Community Structure

为探明添加不同剂量纳米羟基磷灰石对重金属污染土壤的修复效果及其对该土壤微生物群落结构的影响,试验设5个处理,除对照(磷铅摩尔比为0)外,其他4个处理按磷铅摩尔比为0.5、1.0、2.0和4.0分别添加纳米羟基磷灰石,采用BCR连续提取法和BIOLOG微平板法分析。结果显示:培养60 d后,采用BCR连续提取法分析,纳米羟基磷灰石显著增加了各重金属残渣态含量,添加纳米羟基磷灰石后,P/Pb摩尔比越高,非残渣态向残渣态的转化率越高,以P/Pb摩尔比为4.0时转化率最高;采用BIOLOG微平板法分析,添加纳米羟基磷灰石各处理的平均颜色变化率与微生物多样性指数都显著高于对照,表明修复后的土壤微生物群落得到了改良。

To investigate the remediation of heavy metal contaminated soil by different-dose nano-hydroxyapatite（nHAP） and its influence on microbial community,five treaments were designed in this study： control and other four treatments amended with nHAP of different P/Pb molar ratios of 0.5,1.0,2.0 and 4.0,respectively.After 60 d,the results of BCR sequential extraction revealed that nano-hydroxyapatite significantly increased the residual fraction amounts of heavy metals,and the higher the P/Pb molar ratio,the higher the convert ratio of the heavy metals from non-residual fraction to residual fraction.The convert ratio of heavy metals in soil was the highest when the P/Pb molar ratio was 4.0.The analysis of BIOLOG method showed that average well color development（AWCD） and microbial diversity index of microbial community in the treatments amended with nHAP were significantly higher than that of control,which indicated that nHAP amendments effectively repaired the contaminated soils and improved microbial community structure.