ApoE基因多态性与脑瘫并发智力落后的关系

Association between ApoE Genotype and Cerebral Palsy Combined with Mental Retardation

目的:探讨载脂蛋白E(ApoE)基因多态性与中国汉族儿童脑性瘫痪(CP)并发智力落后(Mental Retar-dation,MR)的关系。方法:应用聚合酶链反应与限制性片断长度多态性(PCR-RFLP)分析方法对47例CP并发MR(并发组)、73例CP无MR(无并发组)的患儿与正常儿童(正常组)ApoE基因型和等位基因进行测定,并探讨ApoE基因多态性与CP并发MR的关系。结果:并发组、无并发组及对照组ApoE基因型及其等位基因频率分布均具有统计学意义(P<0.05),ε4等位基因携带患儿频率并发组与无并发组比较,并发组与对照组比较及无并发组与对照组两两比较均具有统计学意义(χ2=5.753、24.667、5.753,P<0.05,0.01,0.05)。结论:ApoEε4等位基因携带患儿频率增高既与CP相关,又可能是CP患者合并MR的一种遗传易感因子。

Objective：To investigate the association between ApoE genotype and cerebral palsy combined with mental retardation （CP-MR）. Methods：Polymerase chain reaction-restricted fragment length polymorphism （PCR-RFLP） was used to determine genotype and allele of ApoE genotype in 47 children with CP-MR （ CP-MR group）,73 children with cerebral palsy not combined with mental retardation （CP-non MR） group and 120 normal children （control group）. Results：There was significant difference in the distribution of the allelic frequency and genotype in ApoE in CP-MR group,CP non-MR group and control group （P=0.000,P=0.000）. There was significant difference in carriage of the ε4 allele between CP-MR group and CP non-MR group,CP-MR group and control group,CP non-MR group and control group （χ2=5.753,24.667,5.753,P0.05,0.01,0.05 ）. Conclusion：Carriage of the ε4 is related with CP and MR.