马铃薯^(60)Co-γ辐射诱变突变体的分子鉴定

Molecular Identification of Potato Mutants Induced by ^(60)Co-γ Radiation

为了对马铃薯辐射诱变突变体的真实性提供分子证据,利用SRAP分子标记技术,对费乌瑞它及其辐射诱变突变体F0802、F0803-Ⅰ、F0803-Ⅱ和F0803-Ⅲ进行分子标记分析。结果表明,亲本与突变体间的遗传相似系数较高,都达到0.842以上;且聚类分析结果与4个突变体来源完全吻合。说明,从分子水平上确定了F0802、F0803-Ⅰ、F0803-Ⅱ和F0803-Ⅲ是费乌瑞它亲本的突变体;SRAP标记技术进行马铃薯辐射诱变突变体分子鉴定是可行的。

The molecular markers of F0802,F0803-Ⅰ,F0803-Ⅱ and F0803-Ⅲ mutants of Favorita radiated by ^60Co-γ and Favorita were analyzed by SRAP molecular marker to provide a molecular evidence for potato mutants.The results showed that the genetic similarity coefficient between the parent and mutants reached above 0.842 and the parent origin accorded with origin of mutants by clustering analysis,which indicates that F0802,F0803-Ⅰ,F0803-Ⅱ and F0803-Ⅲ mutants are really induced from Favorita on the molecular level,and application of SRAP marker technique in molecular identification of mutants induced by ^60Co-γ radiation is feasible.