血小板膜糖蛋白Ⅰbα VNTR基因多态性与脑梗死的相关性研究

Study on the correlation platelet of glycoproteinⅠbα gene polymorphism and cerebral infarction

目的探讨血小板膜糖蛋白(GP)Ⅰb基因α链变数串联重复序列(VNTR)的等位基因和基因型在中国北方人群中的分布规律,并深入研究这种多态性与脑梗死的相关性。方法共收集105例脑梗死病人和100例对照者,用聚合酶链反应和限制性片段长度多态性分析(PCR-RFLP)的方法确定其基因型。结果共发现CC、DD纯合子基因型和CD、BD、BC杂合子基因型,未发现AA基因型。脑梗死组和对照组的BC基因型频率分别为0.067和0.01,B等位基因频率分别为0.057和0.01,差异均有统计学意义(P<0.05)。脑梗死组的BC基因型频率和B等位基因频率显著高于对照组(P=0.036,OR=7.071,95%CI 0.85～58.55;P=0.023,OR=3.98,95%CI 1.106～14.32)。结论血小板膜糖蛋白GPⅠbα(VNTR)基因多态性可能是脑梗死的一个危险因素。

Objective To investigate the relationship between a variable number of tandem repeats(VNTR) polymorphism in platelet glycoprotein Ⅰbα gene and cerebral infarction.Methods One hundred and five cases of cerebral infarction patients and 100 age and sex-matched controls were recruited.The polymorphism was determined by polymerase chain reaction(PCR) and agarose gel electrophoresis.Results The homozygotic CC,DD genotypes and heterozygote CD,BD,BC gene types were found,but no AA.The frequencies of BC genotype were 0.067 and 0.01 in cerebral infarction group and control group respectively,and which of B allele were 0.057 and 0.01,respectively.The differences were all significant(P<0.05).Conclusion The platelet glycoprotein Ⅰbα gene polymorphism may be a risk factor for cerebral infarction.