纳洛酮对铝致学习记忆减退大鼠海马锥体细胞凋亡的影响

Effects of naloxone on apoptosis of hippocampal neurons in rats with aluminum-induced learning and memory impairment

目的观察纳洛酮对铝致学习记忆减退大鼠海马锥体细胞凋亡的影响。方法采用慢性氯化铝灌胃方法,制备学习记忆减退大鼠模型后随机分为模型组和纳洛酮治疗组,另设正常对照组。纳洛酮治疗组大鼠连续7 d腹腔注射纳洛酮。采用脱氧核苷酸末端转移酶介导的脱氧尿苷酸-生物素切口末端标记法,原位检测凋亡细胞,流式细胞仪检测凋亡率。结果纳洛酮组大鼠海马锥体细胞的凋亡率为(6.4±1.6)%,模型组(9.6±1.9)%,差异有统计学意义(P<0.05);纳洛酮组大鼠凋亡细胞数为(7.5±1.9),模型组(12.8±2.2),差异有统计学意义(P<0.05)。结论纳洛酮能减少铝致学习记忆减退大鼠海马锥体细胞的凋亡。

Objective To observe the effects of naloxone on apoptosis of hippocampus pyramidal neurons in rats with aluminum-induced learning and memory impairment. Methods Aluminum-induced learning and memory impairment model was established by gavages of aluminum chloride （600 mg/kg） for 3 months. Rats were divided into three groups,viz, Naloxone trea- ted rats （NAL 0.8 mg/kg, ip daily for 7 days）, nontreated model rats and normal controls. The apoptosis in situ in hippocam- pus pyramidal neurons of the three groups of rats was investigated with a TdT-mediated dUTP nicked labeling （TUNEL） method. The apoptotic rate of neurons was measured by flow cytometry. Results The apoptosis ratio of hippocampus cell in the NAL group （6.4± 1.6）% was significantly decreased compared to nontreated model rats （9.6± 1.9）% （P〈0.05）. The number of apoptotic pyramidal neurons in the CA1 region of hippocampus in the NAL group（7.5 ± 1.9） was significantly decreased compared to nontreated model rats （12.8±2.2） （P〈0.05）. Conclusion Naloxone could decrease the apoptosis of pyramidal neurons in rats with aluminum induced learning and memory impairment.