塞来昔布对人胶质瘤U251细胞增殖及NF-κB蛋白表达的影响

Effect of celecoxib on the proliferation and NF-κB expression of glioma U251 cell

目的观察塞来昔布对人恶性胶质瘤U251细胞增殖的影响及分子机制。方法将对数生长期人胶质瘤U251细胞随机分为塞来昔布组、TNF-α组,两组均分别加入0、10、25、50、100μmol/L的塞来昔布,其中TNF-α组于1h后加50ng/mlTNF-α。其后采用甲基噻唑蓝(MTT)法检测两组U251细胞增殖水平及抑制率,采用免疫组化及Western blot检测NF-κB细胞内分布及核内表达情况。结果塞来昔布浓度为10～100μmol/L时两组细胞增殖抑制率均显著高于浓度为0者且呈浓度依赖性,增殖水平则相反,组间比较无显著差异;塞来昔布浓度为50、100μmol/L时NF-κB蛋白在细胞核内的表达显著低于浓度为0者,且呈浓度依赖性。结论塞来昔布可抑制人胶质瘤U251细胞增殖,可能机制为间接抑制NF-κB向核内移位。

Objective To investigate the effect of celecoxib on the proliferation human malignant glioma and its molec- ular mechanism. Methods Human glioma U251 cells in logarithmic phase were divided into celecoxib group and TNF-α group,both groups were given celecoxib at 0,10,25,50,100 μmol/L,the TNF-α group was given 50 ng/ml TNF-α 1 h later. The cell proliferation level and inhibition ratio were detected by using MTT method,the intracellular distribution and intranuclear expression of nuclear factors（ NF-κB） was measured by immunohistochemistry and western blotting. Results The cell proliferation inhibition ratio increased in the two groups after treated with 10 ～ 100 μmol/L celecoxib and showed a does-dependent manner,the proliferation level was in opposite,no significance was found between the two groups . The intranuclear expression of NF-κB decreased after treated with 50,100μmol/L celecoxib,and also showed a does-dependent man- ner. Conclusion Celecoxib can inhibit the cell proliferation,which may be mediated by inhibiting nuclear translocation of NF-κB indirectly.