猪伪狂犬病病毒蛋白激酶基因的克隆及其部分生物信息学分析

Cloning of protein kinase genes of procine pseudorabies viruses and analyses of their partial bioinformatics

采用PCR方法扩增出12株伪狂犬病病毒（PRV）的蛋白激酶（Protein Kinase,PK）基因全序列,并测序,使用生物信息学软件对这12株PRV的PK基因序列以及GenBank登录的6条PK基因序列进行了生物信息学分析和预测。结果显示,PRV PK基因开放阅读框的核苷酸长度为1 107~1 170 bp,氨基酸长度为368~389个;核酸和氨基酸的同源性分别为96.7%~100%和73.9%~100%;在PK基因核酸序列1 079~1 099 bp和240~250 bp处有2个高变重复区;蛋白质疏水性、抗原表位的分析结果十分相似,而且PK基因编码的蛋白质均具有真核细胞丝氨酸/苏氨酸蛋白激酶催化结构域的保守氨基酸共有序列和亚结构域特征序列。结果表明,PRV PK基因在大部分毒株具有较高的保守性。

The full-length PK genes of 12 strains of pseudorabies virus（PRV） were amplified by PCR,sequenced,analyzed and then predicted with 6 PK gene sequences available in GenBank by bioinformatics software.The open reading frame of the PK gene was 1 107 to 1 164 bp and the length of amino acids was 369 to 388;nucleotide homology was 96.7% to 100%,and amino acids homology was 73.9% to 100%;Two hypermutation replicated plot was located in 1 079-1 099 bp and 240-250 bp.The results of proteo-hydrophilicity and epitope were very similar,and the amino acids of PRV PK contained the most of the conserved motifs of a eukaryotic serine/threonine protein kinase.The result showed that the PK gene was highly conservative,and played an important role in the life cycle of PRV.