隆朋对犬红细胞ATPase活性及其抗氧化功能的影响

Effects of Xylazine on ATPase and antioxidant function of erythrocyte membran in dogs

将12只健康本地犬,肌肉注射隆朋3.0 mg/kg,在注药前(即对照组)及注药后2、8、24、72、120、168 h静脉采血,分别测定红细胞三磷酸腺苷酶(ATPase)、总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量。结果显示,注射隆朋后红细胞膜Na+-K+-ATPase、Ca2+-ATPase、Mg2+-ATPase三种酶活性均呈下降趋势,并在8 h均降到最低值,与对照值比较差异显著(P<0.05)。注射隆朋后2 h T-AOC、SOD活性显著低于对照组(P<0.05);而MDA的含量在注药后2 h高于对照组且差异极显著(P<0.01),120 h基本恢复正常。本试验结果表明,隆朋对于犬红细胞膜Na+-K+-ATPase、Ca2+-ATPase、Mg2+-ATPase活性均有不同程度的抑制作用。隆朋对于犬的T-AOC及SOD的活性存在显著影响,并会引起红细胞分泌MDA含量升高,从而导致红细胞膜发生损伤。

Twelve healthy local dogs were selected in this experiment,they were intramuscularly injected Xylazine 3.0 mg/kg body weight,then vein blood were got before injected and 2,8,24,72,120,168 h after injected.Determined the activities of rhodocyte ATPase,T-AOC,SOD and the concentration of MDA by chromatometry,respectively.The results showed that the activities of Na＋-K＋-ATPase,Ca2＋-ATPase,Mg2＋-ATPase on rhodocyte membran descended,and dropped to the nadir at 8 h,after injected（P0.05）.The activities of T-AOC and SOD were significant lower than the control group（P0.05）.The concentration of MDA 2 h after injected was extremely significant high compared with the control group（P0.01）,and regained to the normal after 120 h.The conclusions were following：there were distinct depressant effects of Xylazine on the activities of Na＋-K＋-ATPase,Ca2＋-ATPase,Mg2＋-ATPase on rhodocyte membran in dogs;there were significant influences of Xylazine on the activities of T-AOC and SOD,and they evoked the increasing concentration of MDA,then resulted in impairment of rhodocyte membran.