H_2S在内毒素致肝损伤中的作用及CA的保护效应

The effects of hydrogen sulfide in endotoxin-induced hepatic injury and protection of Cation A

将72只健康大鼠随机分为生理盐水(NS)对照组、内毒素(ET)致病组、阳离子A(CA)保护组。3组动物经相应处理后分别在3、4、8、12 h采集肝脏作为检测样本,HE染色观察肝脏病理损伤,并采用分光光度法检测肝脏中硫化氢(H2S)含量。结果显示,NS组肝脏组织未见异常,ET组出现微循环障碍与细胞变性坏死等病变,CA保护组则病理变化程度较弱,出现的时间较迟;ET能够使肝脏组织中H2S水平显著升高,而CA保护组H2S在肝脏组织中的含量均显著低于ET组。结果表明,H2S参与了ET致肝脏损伤的病理过程,而CA通过下调肝脏组织中H2S的水平从而对ET的炎性损伤发挥了保护作用。

Seventy-two adult healthy rats were randomly divided into three groups：control group,ET group,CA group.Liver of three groups was collected at 3,4,8 and 12 h after treatment.The alteration of pathological structure in liver was viewed with HE staining.H2S concentration in liver was measured by spectrophotography.Compared with control group,liver in ET group displayed microcirculation disturbance,cellular degeneration and necrosis.In CA group,these alterations were slight and put off compared with ET group.The H2S concentration in liver of ET group was significantly higher than that of control group,while the item in CA group was significantly lower compared with ET group.The above results suggested a novel gaseous transmitter——H2S mediates hepatic injure caused by endotoxin,and CA has a significantly pretective effect on inflammatory injure through down-regulation of H2S level in liver.