凝胶层析分离提纯纳豆激酶研究

Research on Nattokinase Separation and Purification by Gel Chromatography

从纳豆菌发酵液中提取纳豆激酶,采用20%～60%饱和度的硫酸铵沉淀,Supherdex G-75凝胶过滤层析对活性组分进行分离提纯。并用十二烷基磺酸钠-聚丙烯酰胺凝胶电（SDS-PAGE）对分离纯化的效果进行了检验。结果表明在SDS-PAGE中观察到单一条带,分子量27.7ku。

In order to obtain high purity nattokinase from the fermentation broth of Bacillus subtilis,some separation and purification techniques were explored.The optimal separation and purification processing conditions were as follows：removing cells by the centrifugation,20%-60% saturation ammonium sulfateprecipitation,Superdex G-75 gel filtration chromatography.Sodium dodecyl sulfate-polyacrylamide gel electrophoresis（SDS-PAGE）was used toexamine the purification effect,and the results indicate that the final product obtained is homogeneous and has amolecular weight around 27.7ku.The fibrinolytic activity of the nattokinase was measured by means of fibrin plate method.