摘要
目的观察紧密连接蛋白Claudin-2在大肠癌组织中的表达及其与大肠癌发生和发展的关系。方法运用免疫组化技术检测Claudin-2在大肠癌及癌旁组织、正常组织中的表达,并分析Claudin-2蛋白表达与大肠癌临床各病理特征的关系。结果Claudin-2在大肠癌组织中的阳性表达率明显高于癌旁组织及正常组织(P<0.05)。Claudin-2在大肠癌组织中的阳性表达与肿瘤的分化程度、Dukes分期密切相关(P<0.05)。结论大肠癌与癌旁组织及正常组织相比,Claudin-2表达阳性率明显升高,其改变在大肠癌的发生发展中可能起重要作用。
Objective To observe the expression of Claudin-2 in colorectal cancer tissues and to explore its relationship with the development and progression of colorectal cancer.Methods Immunohistochemical staining（SP method） was used to detect the expression of Claudin-2 in colorectal cancer tissue,adjacent normal tissue of colorectal cancer and normal tissue and investigate the correlation between expression of Claudin-2 in colorectal cancer tissue and the genesis and development of colorectal cancer.Results The expression of Claudin-2 in colorectal cancer tissues was significantly higher than those in the adjacent tissues of colorectal cancer and normal colorectal tissue（P〈0.05）;besides,the expression of Claudin-2 in the colorectal cancer tissue was correlated with degree of differentiation,Dukes stages of colorectal cancer（P〈0.05）.Conclusion The expression of Claudin-2 in colorectal cancer increases significantly in both positive rate and intensity compared with that in normal tissue.The higher expression of Claudin-2 may play an important role in the genesis and development of colorectal cancer.
出处
《宁夏医科大学学报》
2010年第5期600-602,F0004,共4页
Journal of Ningxia Medical University
基金
宁夏高等学校科学研究项目(2005)
