摘要
[目的]建立一个紫外可见分光光度法,对不同产地柴胡样品中的多糖进行含量测定。[方法]柴胡多糖含量测定采用苯酚-硫酸显色,487nm为检测波长,对提取过程的主要步骤及方法学进行实验,建立其测定方法;[结果]柴胡多糖的的测定方法简便、准确、稳定,线性范围为10.484～75.936μg,(r=0.9986),11个不同产地的柴胡中多糖含量范围在46.299～96.965g/kg生药;[结论]本研究建立的柴胡多糖含量测定方法简便易行、稳定、可靠。
[Objective] To set up an ultraviolet visible spectrophotometry（UV） method to determine the content of polysaccharide in herbs obtaining from different areas.[Methods] The content of Polysaccharide was tested with the color appearing in the phenol-sulphuricacid reagent.The testing wavelength was 478nm.Its UV assaying method was found by experimenting on the key steps and methodology of extraction.[Results] UV assay method for Polysaccharide was simple and convenient,accurate,and stable.Its linearity range was 10.484～75.936μg.Among the Radix bupleuris from 11 places of origin,the content range of Polysaccharide per 1 gram of crude drug was 46.99～96.965mg.[Conclusion] The method was simple and easy to perform and the obtained results were stable and reliable.
出处
《天津中医药》
CAS
2010年第4期342-343,共2页
Tianjin Journal of Traditional Chinese Medicine
关键词
柴胡多糖
含量测定
紫外可见分光光度法
Polysaccharide
quantitative analysis
ultraviolet visible spectrophotometry
