利用花粉管通道法将抗虫基因(cryV)转入大豆的研究

Introduction of Anti-pest Gene (cry V) to Soybean via Pollen-Tube Pathway

利用花粉管通道法,在大豆的盛花期将外源抗虫基因(cry V)转入到大豆中,转化成活率达43.88%,成荚率达33.6%。将收获的的种子种植在大豆所温室中,通过卡那霉素筛选、PCR鉴定,Southern-blot检测和RT-PCR检测,共检测出5株阳性植株,确定外源抗虫基因(cry V)已转入到大豆中,转化率达1.8‰。

Anti-pest gene（cry V） was introduced to soybean via pollen-tube pathway during blossom period.1211 flowers out of 2760 ones survived 3 days after the operation,the survival rate and podding rate was 43.88%and 33.6%,respectively.The harvested seeds were planted in greenhouse and 5 plants with positive result were obtained after Km screening,PCR identification,southern-blot testing and RT-PCR testing.Results suggestted that the anti-pest gene（cry V） were successfully introduced into those 5 plants and the transformation rate was 1.8‰.