纳米银对大豆菌核病核盘菌的抑制作用研究

Restrain Ability of Nanomenter Silver on Soybean Sclerotinia sclerotrum

利用生长速率抑制法、显微观察和生物化学方法研究了纳米银离子对大豆菌核病核盘菌的抑菌效果及初步机理。结果表明:纳米银水剂对大豆菌核病核盘菌具有较好的抑制能力,在30 mg.L-1浓度下可达到100%的抑制效果,其对大豆菌核病核盘菌的有效中浓度(EC50)为13.91 mg.L-1;菌丝在纳米银有效中浓度下的生长受到很强抑制;经纳米银处理的核盘菌菌体中过氧化氢酶、总超氧化物歧化酶、超微量ATP酶的活力均发生相应变化,处理2 h或12 h后,呈现增加趋势,但处理24 h后,则均表现为下降。因而纳米银对大豆菌核病菌的生长具有一定的抑制效果。

The methods of growth speed inhibition,microexamination and biochemistry were used to study the restrain ability of Nanomenter Silver on Sclerotinia sclerotrum mycelium growth and its primary mechanism.The results indicated that Nanomenter Silver significantly inhibited Sclerotinia sclerotrum mycelium growth,the completely inhibited concentration was 30mg·L-1,and the EC50 was 13.91 mg·L-1.It was discovered that the S.sclerotrum mycelium growth was restrained effectively under the EC50 of Nanomenter Silver.The enzyme activities of CAT,SOD and ATP in mycelium treated by Nanomenter Silver were also changed,when the mycelium were treated for 2 or 12 h,the enzyme activities were all increased,However,when the mycelium were treated for 24 h,the enzyme activities were all decreased.The results suggest that Nanomenter Silver has the restrain ability on the S.sclerotrum mycelium growth.