四氯乙烯污染对大豆幼苗抗氧化特征的影响

Influence of Perchloroethylene on Antioxidation Characteristics of Soybean Seedlings

通过室内模拟试验,研究了不同浓度四氯乙烯(PCE)污染对大豆幼苗抗氧化特性的影响。结果表明:随PCE胁迫强度的增加,大豆幼苗的丙二醛(MDA)、超氧阴离子(O2.-)、脯氨酸及谷胱甘肽(GSH)含量均呈累积升高的趋势,且均存在显著的剂量-效应关系。与对照组相比,当浓度达到0.30 mg.kg-1时,大豆幼苗体内脯氨酸及GSH含量明显累积;浓度达到30.00 mg.kg-1时,O2.-及MDA含量明显升高,幼苗膜脂过氧化显著;低浓度PCE胁迫会激活SOD与CAT酶的活性,当浓度超过0.30 mg.kg-1时则随浓度的升高而降低。

Effects of different perchloroethylene（PCE）concentration on biological indexes of soybeans seedlings were studied by using simulated experiment in laborotary.Results showed the contents of MDA、O-·2,proline and GSH in soybean seedlings all increased as the PCE stress strenthened,and obvious dose-effect relationships between them were found.The proline and GSH contents increased evidently when PCE concentrations were above 0.30 mg·kg-1.When PCE concentrations were above 30.00 mg·kg-1 the O-·2 and MDA contents increased evidently,and the lipid peroxidation of soybean seedlings strengthened.It was also showed that the activity of SOD and CAT were activated in lower PCE stress,and decreased when PCE concentrations were above 0.30 mg·kg-1.