摘要
目的建立高效液相色谱法(high-performance liquid chromatography,HPLC)测定万艾可片中枸橼酸西地那非含量的方法。方法 C18色谱柱(4.6mm×250mm,5μm),以水-甲醇(2∶8)为流动相;流速为1ml/min,检测波长为290nm。结果回归方程为Y=1197.454 X+14.984,r=0.9999,线性范围1.1392～5.6960μg,平均加样回收率为100.08%(RSD=1.36%),样品在12h内稳定。结论本方法结果准确可靠,可用于万艾可片的质量控制。
Objective To establish a method for determining sildenafil citrate contents in Viagra by high-performance liquid chromatography （HPLC）. Methods The chromatographic conditions were： diamonsil C18 column （4.6 mm×250 mm, 5μm）, methanol-water （8∶2） as the mobile phase at a flow rate of 1ml/min and a detective wavelength at 290 nm. Results Sildenafil citrate showed a good linear relationship at the range of 1.1392~5.6960μg, the linear equation was Y=1197.454X＋14.984 （r=0.9999）, the recovery rate was 100.08% （RSD=1.36%） and the sample was stable within 12 hours.Conclusion The method is sensitive, accurate, reproducible, specific and can be efficiently used for quality control of sildenafil citrate in Viagra.
出处
《华南国防医学杂志》
CAS
2010年第4期248-249,共2页
Military Medical Journal of South China
