应用IT-ISJ标记分析加工苹果品种的遗传关系

Application on Genetic Analysis of Processing Apple Varieties Using IT-ISJ Markers

以鲁加1号(Malus domestica Borkh.‘Lujia 1’)等31个加工苹果品种为试材,采用IT-ISJ标记(Intron Targeted Intron-exon Splice Junction,内含子-外显子拼接位点标记)技术,分析了苹果品种的遗传关系。结果表明,供试的31个苹果品种的相似系数平均值为0.49,变异系数为0.49±0.37。其中,倭锦和邦扎相似系数最小,仅为0.26;鲁加2号和鲁加6号相似系数最大,达到了0.86。供试苹果品种中相似系数大于0.50的组合有182个,占供试组合总数的39.3%,而相似系数小于0.50的组合有283个,占供试组合总数的60.7%,说明供试苹果品种之间存在较大的遗传差异。

The experiment was carried out with the technique of IT-ISJ（Intron Targeted Intron-exon Splice Junction） markers on 31 processing apple varieties including Lujia 1（Malus domestica Borkh.＇Lujia 1＇）.The genetic relationships of 31 processing apple varieties were analyzed.It＇s showed that the average of the similarity coefficient among the materials was 0.49,coefficient of variation was 0.49±0.37.The smallest was 0.26 between Ben Davids and Bonza.The biggest similarity coefficient was 0.86 between Lujia 2 and Lujia 6.There were 182 combinations which similarity coefficients were bigger than 0.50,that accounted 39.3% of all the tested combinations.There were 283 combinations which similarity coefficients were smaller than 0.50,that accounted 60.7% of all the tested combinations.The results proved the genetic differences among the materials were big.