云南会泽废弃铅锌矿区和非矿区小花南芥根际真菌的耐镉性

Cadmium Tolerance of Rhizosphere Fungi of Arabis alpina in Abandoned Lead-zinc Mining and Non-mining Area in Huize, Yunnan, China

采用常规、含Cd^(2+)和含Pb^(2+)的马丁氏培养基,对云南省会泽县废弃铅锌矿区和非矿区小花南芥(Arabisalpine)根际真菌进行分离,将分离的菌株接种到含不同浓度(0、0.05、0.5、5mmolL^(-1))Cd^(2+)的马铃薯葡萄糖培养液中,比较废弃铅锌矿区和非矿区小花南芥根际真菌的镉耐性.结果表明:Cd^(2+)显著抑制铅锌矿区和非矿区小花南芥根际真菌的生长;常规、含Cd^(2+)和含Pb^(2+)的马丁氏培养基分离的铅锌矿区小花南芥根际真菌Cd^(2+)的生长半数抑制浓度(EC50值)平均值和最大值均明显大于非矿区,表明铅锌矿区小花南芥根际真菌对Cd^(2+)的耐性强于非矿区;3种培养基分离的真菌中,含Cd^(2+)培养基分离的铅锌矿区小花南芥根际真菌对Cd^(2+)的耐性最强.

Culturable rhizosphere fungi of Arabis alpina in abandoned lead-zinc mining and non-mining area in Huize, Yunnan, China were isolated with normal, Cd^（2＋） and Pb^（2＋） containing Martin’s media. Cd^（2＋） tolerance of those isolated fungi were investigated with potato dextrose fluid media containing 0, 0.05, 0.5 and 5 mmol L^（-1） Cd^（2＋）. The results showed that the growth of rhizosphere fungi of Arenaria orbiculata in the abandoned lead-zinc mining and non-mining area was signif icantly suppressed by Cd^（2＋）. The mean and maximum lethal concentrations of 50% （EC50 value） of Cd^（2＋） to the rhizosphere fungi in the abandoned lead-zinc mining area isolated by normal, Cd^（2＋） and Pb^（2＋） containing media were obviously greater than those in the non-mining area, which indicated that Cd^（2＋） tolerance of the rhizosphere fungi in the abandoned lead-zinc mining area was greater than that in the non-mining area. Among these fungi isolated by these 3 media, the rhizosphere fungi of A. alpina in the abandoned lead-zinc mining area isolated by Cd^（2＋） containing medium were the most tolerant to Cd^（2＋）. Tab3, Ref26