摘要
采用高效液相色谱法测定吉林地区3种乌头中乌头碱、新乌头碱和次乌头碱的含量。结果表明,新乌头碱在0.0016～2.0030g/L范围内线性关系良好,r为0.9996;平均加标回收率为98.6%,相对标准偏差（RSD）1.7%;乌头碱在0.0016～1.0005g/L范围内线性关系良好,r为0.9998;平均加标回收率为97.5%,RSD2.8%;次乌头碱在0.0016～1.0000g/L范围内线性关系良好,r为0.9995;平均加标回收率为96.7%,RSD1.9%。该方法简便易行,结果准确可靠,经对比分析得出,北乌头根中乌头碱（0.1926%）、新乌头碱（0.4658%）和次乌头碱（0.1045%）含量最高,可以作为今后驯化栽培的主要品种。
The contents of three kinds of diester-type alkaloid, namely： aconitine, mesaconitine and hypaconitine in aconitum in Jilin district were measured by high performance liquid chromatography. Results showed that content of mesaconitine has a good linear relationship（r 0.9996）in 0.0016-2.0030g/L with average recovery of 98.6%, relative standard daviation（RSD） 1.7%; The content of aconitine has a good linear relationship（r 0.9998）in 0.0016-1.0005g/L with average recovery of 97.5%, RSD 2.8%; The content of hypaconitine has a good linear relationship（r 0.999 5）in 0.0016-1.0000g/L with average recovery of 96.7%, RSD 1.9%. This method is simple, accurate and reliable, and applicable to quality control of aconitum. From comparative analysis, the contents of aconitine（0.1926%）, mesaconitine（0.4658%） and hypaconitine（0.1045%） were the highest in Aconitum kusnezoffii Reichb. root. This shows that it may be adopted as the main variety of domestication and cultivation in the future.
出处
《林产化学与工业》
EI
CAS
CSCD
北大核心
2010年第4期85-88,共4页
Chemistry and Industry of Forest Products
基金
吉林省林业厅资助项目(吉林科合字第03号)
