山楂叶的化学成分

Chemical constituents from the leaves of the Crataegus pinnatifida Bge.

目的对蔷薇科山楂属植物山楂(Crataegus pinnatifidaBge.)的叶子部分的化学成分进行研究。方法运用硅胶、Sephadex LH-20柱色谱?ODS柱色谱、制备HPLC等分离手段进行化学成分的分离纯化,根据理化性质及波谱数据鉴定其结构。结果从山楂叶体积分数为80%乙醇的提取物中分离得到7个化合物。分别鉴定为18,19-seco,2α,3β,-dihydroxy-19-oxo-urs-11,13(18)-dien-28-oic acid(1)、3,9-dihydroxy-megastigma-5-ene(2)、(3S,5R,6R,7E)-megatsigmane-7-ene-3-hydrox-y-5,6-epoxy-9-O-β-D-glucopyranoside(3)、(Z)-3-hexenyl-6-O-β-D-xylopyranosyl-(1″-6′)-β-D-gluco-pyranoside(β-primeveroside)(4)、苯甲酸(5)、对羟基苯丙酸(6)、反式对羟基桂皮酸(7)。结论化合物1-4为首次从该属植物中分离得到,5-7为首次从该种植物中分离得到。

Objective To study the chemical constituents of the leaves of Crataegus pinnatifida Bge.Methods Seven compounds were isolated by chromatography on silica gel column,Sephadex LH-20,ODS chromatography column,PHPLC and established on the basis of physico-chemical constants and spectroscopic analysis.Results Seven compounds were isolated from 80% ethanol-water extract and their structures were identified as 18,19-seco,2α,3β,-dihydroxy-19-oxo-urs-11,13（18）-dien-28-oic acid（1）,3,9-dihydroxy-megastigma-5-ene（2）,（3S,5R,6R,7E）-megatsigmane-7-ene-3-hydroxy-5,6-epoxy-9-O-β-D-glucopyranoside（3）,（Z）-3-hexenyl-6-O-β-D-xylopyranosyl-（1″-6′）-β-D-glucopyranoside（β-primeveroside）（4）,benzoic acid（5）,p-hydroxy-phenylpropionic acid（6）,trans-p-hydroxy cinamic acid（7）.Conclusions Compounds 14 are isolated from this genus for the first time and compounds 57 are isolated from this plant for the first time.