气相色谱-质谱技术分析香稻特征化合物2-乙酰基吡咯啉

Determination of 2-acetyl-1-pyrroline in aroma rice using gas chromatography-mass spectrometry

建立了气相色谱-质谱技术(GC-MS)分析香稻特征化合物2-乙酰基吡咯啉的方法。在香稻中加入内标物2,4,6-三甲基吡啶,密闭,经无水乙醇-二氯甲烷(体积比为1∶1)在80℃水浴中提取3h。考察了温度和时间对2-乙酰基吡咯啉提取的影响。提取液经毛细管气相色谱柱HP-5MS(30m×0.25mm×0.25μm)分离,全扫描监测模式GC-MS测定。以内标物计,方法的平均回收率为82.57%,相对标准偏差为5.09%,最低检出限为0.01mg/kg。将该方法应用于11份香稻育种材料中2-乙酰基吡咯啉的测定,结果表明,清香米、泰香R207、Texmati、桂香丝糯和中健2号等5个品种中含有2-乙酰基吡咯啉,含量分别为0.097、0.098、0.699、0.045和0.047mg/kg。该方法样品处理简单、快速、灵敏、样本和试剂消耗少,尤其适合于通过测定2-乙酰基吡咯啉含量进行香稻育种前的大批量品种筛选。

A gas chromatography-mass spectrometry method was developed for the determination of 2-ace-tyl-1-pyrroline in aroma rice. 2,4,6-Trimethylpyridine （ TMP） was added into the sample of aroma rice as internal standard,and the compound was extracted by the mixture of anhydrous ethyl alcohol and methylene chloride （ 1∶ 1,v/v） at 80 ℃ for 3 h under sealed condition in water bath. The effects of the temperature and time on the extraction were investigated. The analyte was separated and determined using gas chromatography-mass spectrometry on an HP-5MS capillary column （ 30 m × 0. 25 mm × 0. 25 μm） in scan monitoring mode. Calculated as 2,4,6-trimethylpyridine,the average recovery of the method was 82. 57% with the relative standard deviation of 5. 09%,and the detection limit of method was 0. 01 mg/kg. The method was employed for the determination of 2-acetyl-1-pyrroline in 11 aroma rice breeding varieties. The results showed that 2-acetyl-1-pyrroline was detected in 5 aroma rice varieties,including Qingxiangmi,Taixiang R207,Texmati,Guixiangsinuo and Zhongjian 2,with the contents of 0. 097, 0. 098,0. 699,0. 045 and 0. 047 mg/kg,respectively. The method is simple,rapid and sensitive with low sample and reagent consumption. It is suitable for screening a lot of aroma rice varieties in breeding through the determination of 2-acetyl-1-pyrroline content.