鹅细小病毒不同毒株VP13基因的克隆与序列分析

Cloning and Sequencing of non-repeated VP1 and VP3 Genes of Different Goose Parvovirus Strains

分别将鹅细小病毒(GPV)的5个分离株通过PCR技术,从病毒基因组DNA中扩增出病毒蛋白VP1和VP3非重叠区的基因片段(VP13基因),并与pMD18-T质粒载体连接,转化到感受态大肠埃希菌Top10中,经PCR鉴定后,对插入片段进行序列测定及分析。结果表明,5株GPV VP13基因全长均为594 bp,编码198个氨基酸。不同毒株主要结构蛋白VP13基因与国外已发表的鹅细小病毒B株核苷酸序列相似性较高(93.4%～98.7%),但毒株间也存在一定差异。

In this article,non-repeated VP1 and VP3 genes of different Goose parvovirus strains were amplified from the DNA by（PCR,the non-repeated VP1 and VP3 genes（VP13）were cloned into pMD18-T vector respectively.The recombinant plasmids were identified by PCR and then sequenced.The result of sequence analysis showed that non-repeated VP1 and VP3 gene had 594 bp and included a complete open reading frame which encoding a protein of 198 amino acids,and all the 5 strains of GPV shared highly common identity compared with GPV B strain（93.4%-98.7%）,but there were also some differences between different strains.