前列腺癌细胞系和组织中neprilysin的表达

Expression of neprilysin in prostate cancer cell lines and tissue

目的检测neprilysin(NEP)在前列腺癌细胞系和组织中的表达。方法用实时荧光定量PCR、western blot检测前列腺癌细胞系(LNCaP、PC-3)、39例前列腺癌和16例正常前列腺组织中NEP mRNA和蛋白表达。结果与正常组织相比,LNCaP及PC-3细胞中NEP mRNA表达下调,尤以LNCaP细胞更为显著;在经甲基化抑制剂作用后,PC-3细胞NEP mRNA恢复表达的程度明显高于LNCaP细胞。前列腺癌组织中NEP mRNA和蛋白表达均明显低于正常前列腺。结论 NEP表达下调可能促进前列癌发生。

Objective To observe the expression of neprilysin （NEP） in prostate cancer cell lines and tissue.Methods Expression of NEP mRNA and protein in 2 cell lines of prostate cancer（LNCaP and PC-3）,39 prostatic carcinomas and 16 normal prostates were detected by real-time quantitative PCR and western blot, respectively.Results NEP mRNA expression in LNCaP and PC-3 cells were down-regulated compared with normal tissues,especially in LNCaP cells.Expression of NEP mRNA and protein was significantly lower in prostate cancer than that in normal prostate.Conclusion NEP downexpression could facilitate the development of prostate cancer.