生物碱基及其衍生物的反相离子对色谱行为研究

Study on the Reversed Phase Ion-Pair Chromatographic Behavior of Bilogical Bases and Their Derivatives

本文报道了以含７．５％ＣＨ３ＯＨ（Ｖ／Ｖ），１０ｍｍｏｌ／Ｌ（Ｃ４Ｈ９）４ＮＢｒ，１０ｍｍｏｌ／ＬＫＨ２ＰＯ４－Ｎａ２ＨＰＯ４（ｐＨ５．０）缓冲溶液为流动相，在ＹＷＧＣ１８柱上，反相离子对色谱法分离尿嘧啶、胸腺嘧啶，腺嘌呤、可可碱、尿嘧啶丙酸、茶碱、咖啡因等七种碱基及其衍生物，用于药物、软性饮料、茶叶中茶碱和咖啡因的测定，标准加入回收率在９５．５％～１０４．５％之间。

The separation of seven bases and their deriratives including uracil,thymine,adenine,cacanine,1-propionyloxyuracil,theophylline and caffeine on a YWGC 18 column were reported by rerersed phase ion-pair chromatography with 7.5% CH 3OH(v/v),10mmol/L(C 4H 9) 4NBr,10m mol/L KH 2PO 4-Na 2HPO 4(pH5.0)buffer solution as a mobile phase.The method was used for the determination of theophylline and caffeine in medicine,soft drink and tea,the 95.5%～104.5% recoveries were obtained.