不同氮源对盐生杜氏藻生长和β-胡萝卜素积累的影响

Effects of Three Nitrogen Sources on the Growth and β carotene Accumulation in Dunaliella salina

研究了 Ｎａ Ｎ Ｏ３、 Ｎ Ｈ４ Ｃｌ和脲 ３ 种不同氮源对盐生杜氏藻生长和 β胡萝卜素积累的影响．结果显示，在０．０５～０．５ ｇ／ Ｌ 浓度范围内，较高浓度 Ｎａ Ｎ Ｏ３ 对杜氏藻生长有利． Ｎ Ｈ４ Ｃｌ对杜氏藻的生长有毒害作用，０．２ ｇ／ Ｌ 的浓度即对杜氏藻生长有明显抑制作用，脲在０．１～１．８ ｇ／ Ｌ 的浓度范围内对杜氏藻的生长有促进作用．但当脲浓度达到 ３．０ ｇ／ Ｌ 时，生长速度又有所下降；杜氏藻细胞中β胡萝卜素积累量与培养基中 Ｎａ Ｎ Ｏ３ 浓度成反比．在室外强光照下，以 ０．１ ｇ／ Ｌ 低浓度脲和 Ｎａ Ｎ Ｏ３ 作为氮源，杜氏藻细胞均可积累较高的 β胡萝卜素含量．本文的实验结果提示，低浓度脲有利于杜氏藻细胞生长和 β胡萝卜素积累，可作为良好的氮源．

The effects of three nitrogen sources (KNO 3,NH 4Cl and urea) on the growth and β carotene accumulation in D.salina are studied. The results showed that in the concentration from 0.1 to 0.5 g/L, higher KNO 3 concentration increased the alga growth. NH 4Cl was toxic to the alga. 0.2 g/L NH 4Cl inhibited the alga growth obviously. Urea increased the alga growth in the concentration from 0.1 to 1.8 g/L. However, 3.0 g/L urea decreased the growth. For the accumulation of β carotene in the alga, lower KNO 3 concentration was better. Under high light of out door and 0.1 g/L KNO 3 or urea as nitrogen source, the alga accumulated higher β carotene.Lower urea concentration was good for both D.salina growth and β-carotene accumulation.